Determination of trimethylamine in fish by capillary electrophoresis with electrogenerated tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection

✍ Scribed by Ming Li; Sang Hak Lee

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 142 KB
• Volume: 22
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1006

✦ Synopsis

Abstract

A capillary electrophoresis with electrogenerated chemiluminescence (CE–ECL) method for the determination of trimethylamine (TMA) in fish was studied. In the presence of TMA, ECL from the reaction of analyte and in situ generated tris(2,2′‐bipyridyl)ruthenium(III) [Ru(bpy)~3~^3+^] at electrode surface could be produced. The ECL detection was performed using a Pt working electrode biased at 1.23 V (vs. Ag/AgCl) potential in a 10 mmol/L sodium borate buffer solution, pH 9.2, containing 3 mmol/L Ru(bpy)~3~^2+^. A linear calibration curve (correlation coefficient = 0.9996) was obtained in the range 8 × 10^−5^–4 × 10^−8^ mol/L for TMA concentration. Recoveries obtained were in the range 98.78–101.46%. The method was successfully applied for the assay of TMA in fish, in combination with solid phase extraction (SPE) disks for sample clean‐up and enrichment. Copyright © 2007 John Wiley & Sons, Ltd.