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Triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis

✍ Scribed by YINGHUA XU; YUNQIANG XU; QIMING HOU; RUIFU YANG; SHUMIN ZHANG

Book ID
109344080
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
154 KB
Volume
118
Category
Article
ISSN
0365-5555

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Rapid detection of bordetella pertussis
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✍ S. K. Poddar πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 217 KB πŸ‘ 1 views

## Abstract A polymerase chain reaction (PCR) assay in real‐time for detection of __B. pertussis__ using SYBR green I as the reporter fluorophore and LightCycler instrument (a thermocycler coupled to a fluorescence detection device) was established and evaluated. The amplified amplicon using series