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A real-time PCR assay for the differentiation of Candida species

✍ Scribed by S. Fricke; C. Fricke; C. Schimmelpfennig; C. Oelkrug; U. Schönfelder; R. Blatz; C. Zilch; S. Faber; N. Hilger; M. Ruhnke; A.C. Rodloff

Book ID: 108840930
Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 259 KB
Volume: 109
Category: Article
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2010.04736.x

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## Abstract Real‐time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in‐house developed molecular diagnostic methods are scare. Therefore, we propose a p