Triple Helix Forming Oligonucleotides

✍ Scribed by Claude Hélène (auth.), Claude Malvy, Annick Harel-Bellan, Linda L. Pritchard (eds.)

• Publisher: Springer US
• Year: 1999
• Tongue: English
• Leaves: 295
• Series: Perspectives in Antisense Science 2
• Edition: 1
• Category: Library

✦ Synopsis

Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.

✦ Table of Contents

Front Matter....Pages i-xv
Front Matter....Pages 1-1
The Antigene Strategy: Progress and Perspectives in Selective Gene Silencing....Pages 3-16
Triple-Helix Structure. The Triple-Helix-Forming Oligonucleotide....Pages 17-31
Thermodynamic State Diagrams of Oligonucleotide Triple Helices....Pages 33-47
Imaging of Triplexes by Electron and Scanning Force Microscopy....Pages 49-62
Chemical Modifications of Triple Helix Forming Oligonucleotides....Pages 63-73
Triple Helix Formation with Modified Oligonucleotides....Pages 75-85
Oligonucleotides as Artificial DNases....Pages 87-97
Front Matter....Pages 99-99
Potential Mechanisms of Action....Pages 101-115
Oligonucleotide Uptake and Delivery in Tissue Culture Cells....Pages 117-127
Triple-Stranded Complexes as Antigene Molecules: Transcription Inhibition Ex Vivo ....Pages 129-143
Gene-Targeting Triple Helix Forming Purine Oligonucleotides....Pages 145-157
DNA Triple Helix as a Tool to Regulate Cytokine Gene Expression....Pages 159-166
Genome Modification by Triplex-Forming Oligonucleotides....Pages 167-179
Psoralen-Coupled Oligonucleotides: In Vivo Binding and Repair....Pages 181-192
Structure and Biology of H DNA....Pages 193-222
Triplex-Binding Proteins....Pages 223-231
RNA and Triple Helices....Pages 233-242
Front Matter....Pages 243-243
Triplexes Involving PNA....Pages 245-255
Triple Helix Stabilizing Agents....Pages 257-271
New Targets for Triple Helix Forming Oligonucleotides....Pages 273-284
Front Matter....Pages 243-243
Triplexes and Biotechnology....Pages 285-294
Back Matter....Pages 295-297

✦ Subjects

Biochemistry, general; Pharmacy; Human Genetics