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Trifluoroethanol-Induced Unfolding of Concanavalin A: Equilibrium and Time-Resolved Optical Spectroscopic Studies †

✍ Scribed by Xu, Qi; Keiderling, Timothy A.

Book ID
120162192
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
384 KB
Volume
44
Category
Article
ISSN
0006-2960

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The urea and guanidine hydrochloride (GdnHCl)-induced denaturation of tetrameric concanavalin A (ConA) at pH 7.2 has been studied by using intrinsic fluorescence, 8-anilino-1-naphthalenesulfonate (ANS) binding, far-UV circular dichroism (CD), and size-exclusion chromatography. The equilibrium denatu