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Trapping and imaging of micron-sized embryos using dielectrophoresis

✍ Scribed by Khashayar Khoshmanesh; Nimrod Kiss; Saeid Nahavandi; Clive W. Evans; Jonathan M. Cooper; David E. Williams; Donald Wlodkowic

Book ID: 102186297
Publisher: John Wiley and Sons
Year: 2011
Tongue: English
Weight: 270 KB
Volume: 32
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.201100160

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✦ Synopsis

Abstract

Development of dielectrophoretic (DEP) arrays for real‐time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single‐shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof‐of‐concept application of chip‐based technologies for the analysis of individual embryos trapped under DEP force.

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