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Transverse1H Cross Relaxation in1H–15N Correlated1H CPMG Experiments

✍ Scribed by Rieko Ishima; John M. Louis; Dennis A. Torchia

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
68 KB
Volume
137
Category
Article
ISSN
1090-7807

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✦ Synopsis

Transverse 1 H cross relaxation was observed in Carr-Purcell-Meiboom-Gill (CPMG) experiments by recording 15 N-1 H correlated spectra of amides in HIV protease that was perdeuterated at nonexchangeable sites. Perdeuteration suppresses 1 H-1 H J coupling and improves spectral resolution and sensitivity. Measurements of cross-peak intensities, arising from cross relaxation, were made as a function of (i) ⌬f, the frequency difference between the spins, and (ii) CPMG , one-half of the duration between CPMG pulses. Cross peaks were observed when CPMG was less than 1/(2⌬f), in agreement with theoretical calculations.

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