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Transposon Tn951 (TnLac) is defective and related to Tn3

✍ Scribed by Guy Cornelis; Hans Sommer; Heinz Saedler

Book ID: 104696545
Publisher: Springer
Year: 1981
Tongue: English
Weight: 673 KB
Volume: 184
Category: Article
ISSN: 1617-4615
DOI: 10.1007/bf00272911

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✦ Synopsis

Tn951 is flanked by two perfect inverted repeats of 41 bp which include the 38 bp sequence of the IR of Tn3. Tn951 also contains the last 100 bp of the tnpA gene but with at least two mutations. However, beyond nucleotide 137 the sequences diverge and hybridization experiments show that Tn951 lacks at least the first two thirds of the tnpA gene. In agreement with these observations Tn951 does not transpose by itself at a detectable frequency but can be complemented by the tnpA gene of Tn801 or Tn3. Tn501, Tn1721 and gamma delta do not complement Tn951 transposition. Transposition of Tn951 duplicates 5 bp of target DNA sequence.

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Deletions and an inversion induced by a

Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951

✍ Cornelis, Guy ;Saedler, Heinz 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 837 KB

DNA-DNA filter binding tests, "Southern" blotting experiments and DNA heteroduplex analysis clearly show that Tn951 contains an IS1 element. This IS1-951 sequence is peculiar in that it does not contain the PstI cleavage site which is usually observed on E. coli derived IS1 elements. Nonetheless, IS