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Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951

✍ Scribed by Cornelis, Guy ;Saedler, Heinz

Book ID: 104694940
Publisher: Springer
Year: 1980
Tongue: English
Weight: 837 KB
Volume: 178
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00270486

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✦ Synopsis

DNA-DNA filter binding tests, "Southern" blotting experiments and DNA heteroduplex analysis clearly show that Tn951 contains an IS1 element. This IS1-951 sequence is peculiar in that it does not contain the PstI cleavage site which is usually observed on E. coli derived IS1 elements. Nonetheless, IS1-951 induces deletions. This process is temperature dependent. One instance of an IS1-951 induced inversion was observed, the structure of which is compatible with the current models of transposition of IS elements.

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Multiple integration sites for the lactose transposon Tn951 on plasmid RP1 and establishment of a coordinate system for Tn951

✍ Cornelis, Guy ;Ghosal, Debabrota ;Saedler, Heinz 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 932 KB

Various molecules generated by transposition of the lactose transposon Tn 951 from plasmid pGC1 to plasmid RP1 were examined by DNA heteroduplex and restriction endonuclease analysis. Tn 951 was found to transpose to at least eight different sites on RP 1 in both possible orientations. A coordinate