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Transposon mutagenesis: Cloning of chromosomal DNA from the site of Tn916insertion using polymerase chain reaction

✍ Scribed by Jan Novák; Lea Novak; Ging R. Shah; Wendy A. Woodruff; Page W. Caufield

Book ID
110672548
Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
321 KB
Volume
11
Category
Article
ISSN
0951-208X

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The use of transposon insertion zdc-235:
The use of transposon insertion zdc-235::Tn10 (min 32) to clone and delete DNA from the terminus region of Escherichia coli
✍ Henson, Joan M. ;Kuempel, Peter L. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 846 KB

Transposon zdc-235::Tn10 is inserted at min 32 on the genetic map of Escherichia coli, and we have used this transposon to clone 14 kb of DNA that flanks this insertion. The site of insertion of the transposon, and the restriction map of the cloned DNA, correspond well with the predictions of the Bo