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A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase chain reaction

✍ Scribed by Richard M. Nelson; George L. Long

Book ID
107714689
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
536 KB
Volume
180
Category
Article
ISSN
0003-2697

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Polymerase chain reaction (PCR) amplification was employed to construct a mosaic gene consisting of the propeptide region of protein S and the glutamic acid-rich domain of osteonectin. The strategy is straightforward, results in large amounts of material, and is universally applicable for the genera