Transposon-facilitated DNA sequencing.
โ Scribed by Strathmann, M.; Hamilton, B. A.; Mayeda, C. A.; Simon, M. I.; Meyerowitz, E. M.; Palazzolo, M. J.
- Book ID
- 126764997
- Publisher
- National Academy of Sciences
- Year
- 1991
- Tongue
- English
- Weight
- 1001 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0027-8424
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Improved Vibrio cholerae donors were constructed by introducing the ampicillin transposon, Tn1, into both the conjugative plasmid, P, and the bacterial chromosome to provide "portable regions of homology." The resulting Tfr (Transposon-facilitated recombination) donors transferred genes at high freq
Although the entire DNA sequence of the yeast genome has been determined, the functions of nearly a third of the identified genes are unknown. Recently, we described a collection of mutants, each with a transposon-tagged disruption in an essential gene in Saccharomyces cerevisiae. Identification of