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Transporters involved in regulation of intracellular pH in primary cultured rat brain endothelial cells

โœ Scribed by Caroline J. Taylor; Pieris A. Nicola; Shanshan Wang; Margery A. Barrand; Stephen B. Hladky

Book ID
110115179
Publisher
Cambridge University Press
Year
2006
Tongue
English
Weight
578 KB
Volume
576
Category
Article
ISSN
0022-3751

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Intracellular pH (pH,) and the mechanisms of pH, regulation have been investigated in cultured microglial cells from mouse brain using the pH-sensitive fluorescent dye 2',7'-bis-(2-carboxyethyl)-5-(6)-carboxyfluorescein (BCECF). Cells were acidified by a pulse of NH,+ (4-5 min; 20 mM) and the subseq