The intracellular pH (pHi) of tissue-cultured bovine lens epithelial cells was measured in small groups of 6 to 10 cells using the trapped fluorescent dye 2',7'-bis-(2-,carboxyethyl)-5 (and 6)carboxyfluorescein (BCECF). When perifused at 35 degrees C with artificial aqueous humour solution (AAH) con
Regulation of intracellular pH in cultured bovine retinal pigment epithelial cells
β Scribed by Svea K. Keller; Thomas J. Jentsch; Ilse Janicke; Michael Wiederholt
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 722 KB
- Volume
- 411
- Category
- Article
- ISSN
- 0031-6768
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Membrane conductances for Ca2+ in cultured rat pigment epithelial cells were studied in the whole-cell configuration of the patch-clamp technique using barium (10 mM) as a charge carrier. Two types of voltage-dependent and verapamiland diltiazem-sensitive Ba2+ currents were observed. First, a nearly
Intracellular pH (pH,) and the mechanisms of pH, regulation have been investigated in cultured microglial cells from mouse brain using the pH-sensitive fluorescent dye 2',7'-bis-(2-carboxyethyl)-5-(6)-carboxyfluorescein (BCECF). Cells were acidified by a pulse of NH,+ (4-5 min; 20 mM) and the subseq
## Abstract The purpose of this study was to determine whether angiotensin Iβconverting enzyme (ACE) is present in cultured bovine bronchial epithelial cells (BBECs) and whether its activity can be modulated. We found that extracts of confluent monolayers of cultured BBECs degraded [glycineβ1β^14^C