✦ LIBER ✦

Ca2+-conductances in cultured rat retinal pigment epithelial cells

✍ Scribed by Olaf Strauss; Marion Wienrich

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 700 KB
Volume: 160
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041600111

No coin nor oath required. For personal study only.

✦ Synopsis

Membrane conductances for Ca2+ in cultured rat pigment epithelial cells were studied in the whole-cell configuration of the patch-clamp technique using barium (10 mM) as a charge carrier. Two types of voltage-dependent and verapamiland diltiazem-sensitive Ba2+ currents were observed. First, a nearly sustained current was activated by depolarization to potentials more positive than -30mV and blocked by nifedipine (1 pM). This current was observed in cells of primary cultures less than 13 days old. Second, a transient nifedipine (1 FM) insensitive current was activated by depolarization to potentials more positive than -55mV in cultures which were more than 13 days old. This current was not carried by sodium and blocked by 1 p M tetrodotoxin (TTX). In summary, cultured rat retinal pigment epithelial cells in younger primary cultures express Ba2+ currents indicating the presence of L-type Ca2+ channels. In older primary cultures a low-voltage activated channel was observed with properties different from T-type calcium channels or TTX-sensitive calcium conducting sodium channels.

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