Na+ and Ca2+ channel expression in cultured newt retinal pigment epithelial cells: Comparison with neuronal types of ion channels
✍ Scribed by Sakai, Hideto ;Saito, Takehiko
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 345 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
We cultured retinal pigment epitheinactivation curve tended to be less in cultured RPE lial (RPE) cells dissociated from adult newt eye and cells than in neurons, and the half-inactivation voltage analyzed their voltage-gated ion channels during culwas about 054 mV for the RPE cells and 045 mV for ture using whole-cell patch-clamp techniques. The reneurons. The Ca 2/ current expressed in cultured RPE sults were compared with those of retinal neurons cells was too small to detect without replacement of under identical experimental conditions. After 6-9 external Ca 2/ with Ba 2/ . The Ba 2/ current, like Ca 2/ days in culture (early stage), ú60% of RPE cells decurrent in neurons, was enhanced by Bay-K 8644 and veloped voltage-gated Na / and Ca 2/ channels that blocked by nicardipine. These results suggest that the were not observed in freshly dissociated RPE cells. RPE cells, like neurons, expressed L-type Ca 2/ chan-The number of cells expressing Na / channels and Na / nels in culture. The possibility that the development current density were high after 12-15 days in culture of both Na 2/ and Ca 2/ channels in cultured RPE cells (intermediate stage), while the number of Ca 2/ chanis a manifestation of the transdifferentiation of RPE nel-expressing cells and Ca 2/ current density were cells into neurons is discussed. ᭧ 1997 John Wiley & Sons, high after 20-30 days in culture (late stage). The Inc.