Transport of phenol red in the flounder renal tubule

✍ Scribed by Wasserman, Karlman ;Becker, E. Lovell ;Fishman, Alfred P.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1953
• Tongue: English
• Weight: 421 KB
• Volume: 42
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030420306

✦ Synopsis

O N E FIGURE

Tliis report is coiicerned with the basic meclianisms involved in tlie renal tubular transport of phenol red, as observed in i i a ritro preparations of the floundcr kidney. The procedure is a modification of tliat developed by Forster ('48) and pennits niultiple, sequential microscopic observations on the transport of the dye from the medium across the cell and into tlic lumen.

The particular factors explored were : (1) self-blockade as induced by coiicentrations of phenol red; and (2) the influence of a carbonic anhydrase inhibitor (Diamox or 6063) on acid secretion by the tubule. These observations bear on the bloeliade of tubular transport of p-aminohippuric acid ( P A H ) and dioclrast a t high plasma concentrations in mammals, as revealed by self-depression of Tm and reported by Eggleton and Habib ( 'ZO), Barclay, Cooke and DeMuralt ( '49), and Schachter and Freinkel ('5l), and particularly on the intracellular accumulation of diodrast in the rabbit kidney during overload, as reported by Josephson and his co-workers ('49, 'r>O, ' 5 3 ) .