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Transient induction of cytochrome P450 1A1 mRNA by culture medium component in primary cultures of adult rat hepatocytes

✍ Scribed by Thomas A. Kocarek; Erin G. Schuetz; Philip S. Guzelian


Book ID
112803625
Publisher
Society for In Vitro Biology
Year
1993
Tongue
English
Weight
679 KB
Volume
29
Category
Article
ISSN
1475-2689

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In this investigation, we examined the effects of insulin on gene induction responsiveness in primary rat hepatocytes. Cells were cultured for 72 hours either in the absence or presence of 1 lM insulin and then exposed to increasing concentrations of phenobarbital (PB; 0.01-3.5 mM). Culturing in the