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Dependence of the induction of cytochrome P-450 by phenobarbital in primary cultures of adult rat hepatocytes on the composition of the culture medium

✍ Scribed by Nancy A. Turner; Henry C. Pitot


Book ID
115781104
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
578 KB
Volume
38
Category
Article
ISSN
0006-2952

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In this investigation, we examined the effects of insulin on gene induction responsiveness in primary rat hepatocytes. Cells were cultured for 72 hours either in the absence or presence of 1 lM insulin and then exposed to increasing concentrations of phenobarbital (PB; 0.01-3.5 mM). Culturing in the