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Transient generation of displaced single-stranded DNA during nick translation

✍ Scribed by Olivera, Baldomero M.; Lundquist, Ronald C.


Book ID
123242769
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
789 KB
Volume
31
Category
Article
ISSN
0092-8674

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Determination of DNA single-strand break
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The detection of DNA single-strand breaks (SSB) in human mononucleated white blood cells (MWBC) using a modified version of the nick translation assay is presented. This assay allows rapid and sensitive examination of SSB using only 5 ml heparinized blood fo'~ an eightfold determination. The assay w