✦ LIBER ✦
Determination of DNA single-strand breaks in lymphocytes of smokers and nonsmokers exposed to environmental tobacco smoke using the nick translation assay
✍ Scribed by Einhaus, M. ;Holz, O. ;Mei�ner, R. ;Krause, T. ;Warncke, K. ;Held, I. ;Scherer, G. ;Tricker, A.R. ;Adlkofer, F. ;R�diger, H.W.
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 636 KB
- Volume
- 72
- Category
- Article
- ISSN
- 1432-1440
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✦ Synopsis
The detection of DNA single-strand breaks (SSB) in human mononucleated white blood cells (MWBC) using a modified version of the nick translation assay is presented. This assay allows rapid and sensitive examination of SSB using only 5 ml heparinized blood fo'~ an eightfold determination. The assay was standardized by incubation of