We have constructed a recombinant SV40-based vector carrying the S gene coding for the hepatitis B virus surface antigen (HBsAg). This vector replicates as an episome in monkey COS7 cells, producing high levels of hepatitis B virus surface antigen (HBsAg), which is liberated in the cell medium, prob
✦ LIBER ✦
Transient expression of heterologous model gene in plants usingPotato virusX-based vector
✍ Scribed by Noemi Čeřovská; Tamara Pečenková; Tomáš Moravec; Jiří Velemínský
- Publisher
- Springer
- Year
- 2004
- Tongue
- English
- Weight
- 108 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0167-6857
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A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-[3-glucuronidase (Ubi