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Transient expression of hepatitis B virus surface antigen (HBsAg) gene in monkey cells by a SV40-based virus vector

✍ Scribed by Soraia A. C. Jorge; Maria Concepcion Hera; Angela M. M. Spina; João Renato R. Pinho; Carlos F. M. Menck

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
287 KB
Volume
17
Category
Article
ISSN
0141-5492

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✦ Synopsis

We have constructed a recombinant SV40-based vector carrying the S gene coding for the hepatitis B virus surface antigen (HBsAg). This vector replicates as an episome in monkey COS7 cells, producing high levels of hepatitis B virus surface antigen (HBsAg), which is liberated in the cell medium, probably as a membrane vesicule. The vector also carries the SV40-1ate genes and produces recombinant viruses. These viruses were used to infect fresh cell culture, with detection of HBsAg in the medium. Thus, this virus vector can efficiently transduce the gene for HBsAg.

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