✦ LIBER ✦

Transient expression assay for qualitative assessment of gene expression by fowlpox virus

✍ Scribed by Shree Dhawale; Christopher E. Beisel; Keyvan Nazerian

Publisher: Springer
Year: 1990
Tongue: English
Weight: 485 KB
Volume: 3
Category: Article
ISSN: 0920-8569
DOI: 10.1007/bf00393181

No coin nor oath required. For personal study only.

✦ Synopsis

A transient expression assay for fowlpox virus (FPV) was developed to assess the feasibility of using heterologous promoters in FPV and to qualitatively determine relative promoter strength. A transient expression system for FPV has not been reported, and various methods used for transient expression in vaccinia-virusinfected cells produced negative results when used with FPV. Here a successful method for transient expression of E. coli beta-galactosidase in FPV-infected chick embryo fibroblasts is reported. This transient expression assay has been developed to qualitatively assess promoter recognition and gene expression by FPV. It should also prove useful in the identification of promoters from the FPV genomic library and in testing the accuracy of chimeric promoter-gene constructs.

📜 SIMILAR VOLUMES

A bi-functional reporter plasmid for the

A bi-functional reporter plasmid for the simultaneous transient expression assay of two herpes simplex virus promoters

✍ W. Michael Flanagan; Edward K. Wagner 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 616 KB

Transient expression of hepatitis B viru

Transient expression of hepatitis B virus surface antigen (HBsAg) gene in monkey cells by a SV40-based virus vector

✍ Soraia A. C. Jorge; Maria Concepcion Hera; Angela M. M. Spina; João Renato R. Pi 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 287 KB

We have constructed a recombinant SV40-based vector carrying the S gene coding for the hepatitis B virus surface antigen (HBsAg). This vector replicates as an episome in monkey COS7 cells, producing high levels of hepatitis B virus surface antigen (HBsAg), which is liberated in the cell medium, prob

A Polymerase Chain Reaction-Based Method

A Polymerase Chain Reaction-Based Method for Detection and Quantification of Reporter Gene Expression in Transient Transfection Assays

✍ M.J. Morales; D.I. Gottlieb 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 583 KB

Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt

A Reporter Gene Assay for High-Throughpu

A Reporter Gene Assay for High-Throughput Screening of G-Protein-Coupled Receptors Stably or Transiently Expressed in HEK293 EBNA Cells Grown in Suspension Culture

✍ Yves Durocher; Sylvie Perret; Eric Thibaudeau; Marie-Helene Gaumond; Amine Kamen 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 184 KB

We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us