Transient electrophoretic and velocity sedimentation analysis of lymphocytes

✍ Scribed by Nicholas Catsimpoolas; Ann L. Griffith

• Book ID: 102984080
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 987 KB
• Volume: 80
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90679-0

✦ Synopsis

A new instrument system (TmNs-analyzer) and procedures are described for the separation and characterization of lymphocytes by transient-state electrophoresis (TRANS-EL), isoelectric focusing (TRANS-IF),

and velocity sedimentation at lg (TRANS-VELS) in iso-osmolar density gradients. Monitoring of cell migration in the electrical and lg gravitational fields is performed in situ and repetitively by "absorbance" (light extinction) and scattering measurements. Data acquisition, processing, and display are performed by a computer. Kinetic analysis of the cell distribution provides precise information on the electrophoretic mobility and sedimentation velocity of lymphocytes under standardized conditions. Selective applications of these methods to model systems involving mouse (C57BL16J) thymus and spleen lymphocytes and human peripheral lymphocytes are reported. The availability of these techniques provides a new approach to immunophysical studies concerning the surface charge and hydrodynamic properties of immunocompetent cells.