Transient velocity sedimentation at unit gravity of human erythrocytes

✍ Scribed by S.N. Agathos; A.L. Griffith; R. Uauy-Dagach; V.R. Young; N. Catsimpoolas

• Book ID: 102628097
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 352 KB
• Volume: 81
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90607-8

✦ Synopsis

A new biophysical method, transient velocity sedimentation (TRANS-VELS) at unit gravity, has been applied to the study of human erythrocytes from normal subjects and from patients with diseased states producing abnormal red blood cells. The hydrodynamic behavior of the cells during sedimentation was examined by repetitively recording the distribution profile as a function of time by the use of optical scanning methods. Computer analysis of the distributions allowed precise measurement of the mean sedimentation velocity (S) and the mean skewness (+s ) of peak profile. A two-dimensional plot of s versus 3 indicated significant differences between " normal" and "abnormal" erythrocytes by linear categorization techniques and probability density ellipses at the 0.1 density level. It is concluded that precise measurement of these two new parameters offers a new and valuable approach to the physical characterization of erythrocytes.