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Transient electric birefringence study of the length and stiffness of short DNA restriction fragments

✍ Scribed by John G. Elias; Don Eden

Publisher: Wiley (John Wiley & Sons)
Year: 1981
Tongue: English
Weight: 580 KB
Volume: 20
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1981.360201107

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✦ Synopsis

Abstract

Transient electric birefringence measurements of the rotational diffusion constant of five short restriction fragments of the plasmid pBR322 show that the hydrodynamic length is independent of sodium ion concentration in the range of 0.2 to 2.5 m__M__. The fragments are too stiff to be modeled as wormlike molecules. The rotational relaxation times of the fragments, which range from 64 to 124 base pairs, have been used to calculate the rise per base pair using six different theoretical expressions for the length dependence of the rotational diffusion coefficient of straight cylinder. The best estimate for the rise per base pair of Na‐DNA in solution is 3.3 ± 0.1 Å.

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