๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Rotational diffusion of short DNA fragments in polyacrylamide gels: An electric birefringence study

โœ Scribed by Sybren S. Wijmenga; Anthony Maxwell

Publisher
Wiley (John Wiley & Sons)
Year
1986
Tongue
English
Weight
901 KB
Volume
25
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

โœฆ Synopsis

Using electric birefringence we have examined the rotational diffusion of five short DNA fragments (55 to 256 base pairs) both in polyacrylamide gels as a function of gel concentration and in solution. The length dependence of the measured rotational relaxation times in the gels is in good agreement with the prediction from the Odijk theory for the dynamics of slightly flexible rods in a network. The rotational relaxation times were found to depend on the gel concentration, contrary t o the prediction from the Odijk theory. Possible reasons for this observation are discussed. The birefringence decay curves for DNA fragments in the gel were single exponential only at small electric field strength.

๐Ÿ“œ SIMILAR VOLUMES

Transient electric birefringence study o
Transient electric birefringence study of the length and stiffness of short DNA restriction fragments
โœ John G. Elias; Don Eden ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 580 KB

## Abstract Transient electric birefringence measurements of the rotational diffusion constant of five short restriction fragments of the plasmid pBR322 show that the hydrodynamic length is independent of sodium ion concentration in the range of 0.2 to 2.5 m__M__. The fragments are too stiff to be