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Transient and stable transfection of Leishmania by particle bombardment

✍ Scribed by Sandro Sbicego; Achim Schnaufer; Beat Blum

Book ID
114146530
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
83 KB
Volume
94
Category
Article
ISSN
0166-6851

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Stable transformation of alfalfa (Medica
Stable transformation of alfalfa (Medicago sativaL.) by particle bombardment
✍ L. Filipe Pereira; Larry Erickson πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 410 KB

Stable transformation of alfalfa (Medicago sativa L.) was obtained by particle bombardment of calli (derived from petiole and stem sections cultured for three weeks on SH medium), followed by delayed selection with 50 mg/l kanamycin on BOi2Y medium. Selection at a lower level of kanamycin (25 mg/l)