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Stable transformation of Eustoma grandiflorum by particle bombardment

✍ Scribed by M. Takahashi; M. Nishihara; S. Yamamura; S. Nishizawa; K. Irifune; H. Morikawa

Publisher
Springer
Year
1998
Tongue
English
Weight
951 KB
Volume
17
Category
Article
ISSN
0721-7714

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✍ L. Filipe Pereira; Larry Erickson πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 410 KB

Stable transformation of alfalfa (Medicago sativa L.) was obtained by particle bombardment of calli (derived from petiole and stem sections cultured for three weeks on SH medium), followed by delayed selection with 50 mg/l kanamycin on BOi2Y medium. Selection at a lower level of kanamycin (25 mg/l)