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Stable genetic transformation ofLarix gmeliniiL. by particle bombardment of zygotic embryos

✍ Scribed by Xiaofei Lin; Wenbo Zhang; Katsuaki Takechi; Susumu Takio; Kanji Ono; Hiroyoshi Takano

Publisher
Springer
Year
2005
Tongue
English
Weight
364 KB
Volume
24
Category
Article
ISSN
0721-7714

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✍ L. Filipe Pereira; Larry Erickson πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 410 KB

Stable transformation of alfalfa (Medicago sativa L.) was obtained by particle bombardment of calli (derived from petiole and stem sections cultured for three weeks on SH medium), followed by delayed selection with 50 mg/l kanamycin on BOi2Y medium. Selection at a lower level of kanamycin (25 mg/l)