TransgenicArabidopsis thalianaplants obtained by particle-bombardment-mediated transformation

✍ Scribed by Motoaki Seki; Naoki Shigemoto; Yoshibumi Komeda; Jun Imamura; Yasuyuki Yamada

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 590 KB
• Volume: 36
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00164425

✦ Synopsis

Stable t r a n s f o r m a t i o n o f Arabidopsis thaL iana with p l a s m i d D N A ( p C a M V N E O ) h a r b o u r i n g the n e o m y c i n p h o s p h o t r a n s f e r a s e II (npt-I1) gene was a c h i e v e d using a p r e v i o u s l y d e s c r i b e d p n e u m a t i c particle gun device driven by c o m p r e s s e d air. T r a n s g e n i c A. thaliana p l a n t s were r e g e n e r a t e d f r o m root sections b o m b a r d e d with D N A -c o a t e d gold particles accelerated by the device. E n z y m e assays a n d S o u t h e r n blot h y b r i d i z a t i o n c o n f i r m e d the e x p r e s s i o n o f the foreign gene a n d its stable integration into the Arabidopsis genome. The analysis for k a n a m y c i n resistance in R1 plants f r o m a s e l f -p o l l i n a t e d t r a n s f o r m a n t i n d i c a t e d t r a n s m i s s i o n o f the npt-H gene to R1 p r o g e n y .