β Scribed by Luis Herrera-Estrella, June Simpson, Miguel MartΓnez-Trujillo (auth.), Leandro PeΓ±a (eds.)
No coin nor oath required. For personal study only.
A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular BiologyΠ²βΡ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Front Matter....Pages 3-31
Front Matter....Pages 33-33
Front Matter....Pages 35-46
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Back Matter....Pages 103-109
....Pages 111-137
Plant Sciences
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<p><p>In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In<i> Transgenic Plants: Methods and Protocols, Second Edition </i>expert researchers in the field provi
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