Transgenic Mouse: Methods and Protocols
β Marten H. Hofker (auth.), Marten H. Hofker, Jan van Deursen (eds.)
π Library
π
2002
π Humana Press
π English
β¦ LIBER β¦
π
Transgenic Mouse: Methods and Protocols
β Scribed by Melissa A. Larson
- Publisher
- Springer US;Humana
- Year
- 2020
- Tongue
- English
- Leaves
- 241
- Series
- Methods in Molecular Biology 2066
- Edition
- 1st ed. 2020
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field.
β¦ Table of Contents
Front Matter ....Pages i-x
The History of Transgenesis (Thomas L. Saunders)....Pages 1-26
Pronuclear Microinjection of One-Cell Embryos (Melissa A. Larson)....Pages 27-33
Integrase-Mediated Targeted Transgenics Through Pronuclear Microinjection (Ruby Yanru Chen-Tsai)....Pages 35-46
In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos (Melissa A. Larson, Katelin A. Gibson, Jay L. Vivian)....Pages 47-57
Targeted Mutations in the Mouse via Embryonic Stem Cells (Marina Gertsenstein, Joffrey MiannΓ©, Lydia Teboul, Lauryl M. J. Nutter)....Pages 59-82
Blastocyst Microinjection with Embryonic Stem Cells (Melissa A. Larson)....Pages 83-88
Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos (Bin Gu, Marina Gertsenstein, Eszter Posfai)....Pages 89-100
Embryo Transfer Surgery (Melissa A. Larson)....Pages 101-106
Nonsurgical Embryo Transfer Protocol for Use with the NSETβ’ Device (Barbara J. Stone)....Pages 107-111
Nuclear Transfer and Cloning (Ling Liu)....Pages 113-124
Sperm-Mediated Genetic Modifications (Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, Roberto Giovannoni)....Pages 125-132
Genotyping Genetically Modified (GM) Mice (Neeraj K. Aryal, Jan Parker-Thornburg)....Pages 133-148
Nomenclature: Naming Your Gene-Modified Mouse (Alicia Valenzuela)....Pages 149-162
Breeding Strategies for Genetically Modified Mice (Jan Parker-Thornburg)....Pages 163-169
Strategies for Behaviorally Phenotyping the Transgenic Mouse (Kenneth E. McCarson)....Pages 171-194
Cryobanking and Recovery of Genetically Modified Mice (Toru Takeo, Naomi Nakagata)....Pages 195-209
Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques (Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata)....Pages 211-216
Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus (Julia M. Draper, Jay L. Vivian)....Pages 217-234
Back Matter ....Pages 235-236
β¦ Subjects
Biomedicine; Human Genetics; Animal Models; Stem Cells
π SIMILAR VOLUMES
Transgenic Mouse Methods and Protocols
β Marten H. Hofker (auth.), Marten H. Hofker, Jan van Deursen (eds.)
π Library
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2011
π Humana Press
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<p>The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods an
Transgenic Mouse: Methods and Protocols
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Transgenic Mouse Methods and Protocols
β Marten H. Hofker (auth.), Marten H. Hofker, Jan van Deursen (eds.)
π Library
π
2011
π Humana Press
π English
<p>The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods an
Transgenic Mouse: Methods and Protocols
β Melissa A. Larson
π Library
π
2020
π Human
π English
Transgenic Mouse: Methods and Protocols
β Marten H. Hofker (auth.), Marten H. Hofker, Jan van Deursen (eds.)
π Library
π
2002
π Humana Press
π English
Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigat