Transgenesis Techniques: Principles and
โ David Murphy, David A. Carter
๐ Library
๐
1993
๐ Humana Press
๐ English
โฆ LIBER โฆ
๐
Transgenesis Techniques: Principles and Protocols
โ Scribed by Jim McWhir (auth.), Alan R. Clarke (eds.)
- Publisher
- Springer New York
- Year
- 2002
- Tongue
- English
- Leaves
- 345
- Series
- Methods in Molecular Biology 180
- Category
- Library
โฌ Acquire This Volume
No coin nor oath required. For personal study only.
โฆ Synopsis
The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.
Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.
โฆ Table of Contents
Front Matter....Pages i-x
Front Matter....Pages 1-23
Front Matter....Pages 3-23
Front Matter....Pages 25-36
Front Matter....Pages 27-36
Front Matter....Pages 37-70
Front Matter....Pages 39-70
Front Matter....Pages 71-89
Front Matter....Pages 73-82
Front Matter....Pages 83-90
Back Matter....Pages 91-204
....Pages 93-126
โฆ Subjects
Cell Biology
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Transgenesis Techniques: Principles and
โ Jim McWhir (auth.), Alan R. Clarke (eds.)
๐ Library
๐
2002
๐ Springer New York
๐ English
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