Transgenesis Techniques: Principles and Protocols

✍ Scribed by Jim McWhir (auth.), Alan R. Clarke (eds.)

• Publisher: Springer New York
• Year: 2002
• Tongue: English
• Leaves: 346
• Series: Methods in Molecular Biology 180
• Edition: 2nd ed
• Category: Library

✦ Synopsis

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

✦ Table of Contents

Front Matter....Pages i-x
Front Matter....Pages 1-23
Front Matter....Pages 3-23
Front Matter....Pages 25-36
Front Matter....Pages 27-36
Front Matter....Pages 37-70
Front Matter....Pages 39-70
Front Matter....Pages 71-89
Front Matter....Pages 73-82
Front Matter....Pages 83-90
Back Matter....Pages 91-204
....Pages 93-126

✦ Subjects

Cell Biology