โ Scribed by Philip H. Howe
No coin nor oath required. For personal study only.
Philip H. Howe and a group of well-versed experimentalists present the first major volume a collection of indispensable classic and cutting-edge TGFร assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFร to methods for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature. Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFร induces and modulates physiological behavior.
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Abbreviations: TGF- \(\beta\), Transforming Growth Factor-Beta; TGF- \(\alpha\), Transforming Growth Factor-Alpha; EGF, Epidermal Growth Factor; ECM, Extracellular Matrix; LAP, Latency Associated Protein; CAM, Chorio-Allantoic Membrane; PBN, Peripheral Blood Neutrophil; 5-FU, 5-Fluorouracil; 4-HC, 4