NIH 3T3 cells were transfected with the DNAs from biopsy specimens of human nasopharyngeal carcinoma (NPC, EBV DNA positive) using calcium phosphate precipitation method. The malignant, transformed foci of NIH 3T3 cells have been observed and cloned. The hybridization of transfectant DNA digested by
Transforming activity of nasopharyngeal carcinoma DNA detectable in mouse JB6 cells
β Scribed by N. H. Colburn; N. Raab-Traub; D. Becker; Y. Cao; D. Winterstein
- Book ID
- 102864098
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 961 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0020-7136
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β¦ Synopsis
Publication of the International Union Against Cancer Publication de I'Union lnternationale Contre le Cancer 'To whom reprints should be addressed.
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## Abstract We transformed JB6P+ cells with prolonged intermittent lowβdose UVB radiation or prolonged exposure to lowβdose H~2~O~2~ or CdCl~2~. Stable transformation was confirmed by an anchorageβindependence assay. The JB6P+ transformants formed more colonies (βΌsix folds) in soft agar as compared
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A salt-dependent DNA polymerase activity was demonstrated in the culture of an EBV-producing, lymphoblastoid cell line (NPC-204 cells) treated with 5-iodo-2'-deoxyuridine (IUdR). There was a high frequency of levels of antibody to this enzyme in sera of patients with nasopharyngeal carcinoma (NPC).