𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Transformation ofVicia narbonensisviaAgrobacterium-mediated gene transfer

✍ Scribed by Thomas Pickardt; Martin Meixner; Verena Schade; Otto Schieder

Book ID
104674969
Publisher
Springer
Year
1991
Tongue
English
Weight
697 KB
Volume
9
Category
Article
ISSN
0721-7714

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✦ Synopsis

Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. After transfer to regeneration-medium these calluses produced hygromycin-resistant and nopaline-positive somatic embryos which could be regenerated to plantlets. The integration of the T-DNA into the plant genome was confirmed by Southern analysis.

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