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Agrobacterium-mediated transformation ofVicia faba

✍ Scribed by Petra Böttinger; Anke Steinmetz; Otto Schieder; Thomas Pickardt

Book ID: 110323362
Publisher: Springer Netherlands
Year: 2001
Tongue: English
Weight: 316 KB
Volume: 8
Category: Article
ISSN: 1380-3743
DOI: 10.1023/a:1013711210433

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Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants pr