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Transformation ofSalmonella typhimuriumTA1538 by optimized electroporation

✍ Scribed by Michèle Gentil; Nadine Azas; Michel Méo; Michèle Laget; Gérard Duménil

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
392 KB
Volume
7
Category
Article
ISSN
0951-208X

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✦ Synopsis

Standard electroporation of Salmonella typhimurium TA1538 by the plasmid pAMH70 yielded 1.3~10~ transformantslpg of plasmid DNA. Three parameters: resistance (U,), voltage (U,) and dose of plasmid DNA (U,) were optimized with two Doehlert designs. Transformation was increased 75 fold. Optimal values for Ur, U, and U, were 761.0 Q, 1.6 kV and 96.1 ng respectively. The most important parameters were U2 and the couple U1/U2.

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