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Optimized transformation by electroporation ofLactococcus lactisIL1403

✍ Scribed by Woo-Jin Kim; Tae-Ryeon Heo; Jae-Seong So

Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
406 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

Rolling circle replication

of plasmid pGKV21 in L. luctis was a more stable replication mode than the theta replication of plasmid pIL253.

When the plasmid pGKV21 was used to develop and optimize transformation of L. fsctis by electroporation, optimum transformation was obtained using dense suspension of late-exponential phase cells subjected to a high voltage (12.5 kV/cm) and a short pulse time (0.5 ms) in the presence of a plasmid DNA.

With plasmid vector pGKV21, a transformation frequency of 2.2 X lo8 transformants per fig of DNA was obtained.

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