Transformation ofBotrytis cinereawith the hygromycin B resistance gene,hph
β Scribed by W. Hamada; Ph. Reignault; G. Bompeix; M. Boccara
- Book ID
- 104716252
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 894 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The phytopathogenic fungus Septoria nodorum has been transformed using a plasmid (pAN7-1) containing the Escherichia coli hygromycin phosphotransferase gene (hph). Large, stable hygromycin-resistant transformant colonies appeared at frequencies between 2 and 25 per pg DNA when wheat-adapted and barl
Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria rnaculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression