Transformation of the rice blast fungusMagnaporthe griseato hygromycin B resistance
β Scribed by Hei Leung; Ulla Lehtinen; Reijo Karjalainen; Daniel Skinner; Paul Tooley; Sally Leong; Albert Ellingboe
- Book ID
- 104727358
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 513 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0172-8083
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The phytopathogenic fungus Septoria nodorum has been transformed using a plasmid (pAN7-1) containing the Escherichia coli hygromycin phosphotransferase gene (hph). Large, stable hygromycin-resistant transformant colonies appeared at frequencies between 2 and 25 per pg DNA when wheat-adapted and barl
Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria rnaculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression