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Transformation of the phytopathogen Mycosphaerella graminicola to carbendazim and hygromycin B resistance

✍ Scribed by A. C. Payne; Marie-Claire Grosjean-Cournoyer; Derek W. Hollomon


Book ID
106006292
Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
142 KB
Volume
34
Category
Article
ISSN
0172-8083

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The phytopathogenic fungus Septoria nodorum has been transformed using a plasmid (pAN7-1) containing the Escherichia coli hygromycin phosphotransferase gene (hph). Large, stable hygromycin-resistant transformant colonies appeared at frequencies between 2 and 25 per pg DNA when wheat-adapted and barl