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Transformation of yeast without the use of foreign DNA

✍ Scribed by Morten C. Kielland-Brandt; Torsten Nilsson-Tillgren; Steen Holmberg; Jens G. Litske Petersen; Bo A. Svenningsen


Book ID
105651228
Publisher
Springer-Verlag
Year
1979
Weight
870 KB
Volume
44
Category
Article
ISSN
0105-1938

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Transformation of yeast spheroplasts wit
✍ Peter M.J. Burgers; Kimberly J. Percival πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 598 KB

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased lo-to IOO-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X lo7 and 5 X IO6 transformants/lg, respecti