Transformation of yeast spheroplasts wit
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Peter M.J. Burgers; Kimberly J. Percival
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Article
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1987
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Elsevier Science
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English
β 598 KB
The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased lo-to IOO-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X lo7 and 5 X IO6 transformants/lg, respecti