𝔖 Bobbio Scriptorium
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Transformation of yeast spheroplasts without cell fusion

✍ Scribed by Peter M.J. Burgers; Kimberly J. Percival

Book ID
102987616
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
598 KB
Volume
163
Category
Article
ISSN
0003-2697

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✦ Synopsis

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased lo-to IOO-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X lo7 and 5 X IO6 transformants/lg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 370, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts. 0 1987 Academic press, I~C.

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