Transformation of vitamin D3to 1α,25-dihydroxyvitamin D3via 25-hydroxyvitamin D3usingAmycolatasp. strains

✍ Scribed by Joji Sasaki; Akiko Miyazaki; Mika Saito; Takashi Adachi; Kazutoshi Mizoue; Kazunori Hanada; Sadafumi Omura

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 580 KB
• Volume: 38
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00174460

✦ Synopsis

To enzymatically synthesize active metabolites of vitamin D3, we screened about 500 bacterial strains and 450 fungal strains, of which 12 strains were able to convert vitamin D 3 to lc~,25-dihydroxyvitamin D3 [lc~,25(OH)2.D3] via 25-hyroxyvitamin D3 [25(OH)Da]. The conversion activity was only detected in strains belonging to the genus Amycolata among all the organisms tested. A preparative-scale conversion of vitamin D~ to 25(OH)D3 and lot,25(OH)2D3 in a 200-1 tank fermentor using A. autotrophica FERM BP-1573 was accomplished, yielding 8.3 mg 25(OH)D3/1 culture and 0.17 mg l~,25(OH)2D3/1 culture. A related compound, vitamin D2, could be also converted to 25-hydroxyvitamin D2 and la,25-dihydroxyvitamin D2 using the same strain. The cytochrome P-450 of FERM BP-1573 was detected by reduced CO difference spectra in wholecell suspensions. Vitamin Da in the culture induced cytochrome P-450 and the conversion activity simultaneously, suggesting that the hydroxylation at C-25 of vitamin D3 and at C-1 of 25(OH)D3 originates from cytochrome P-450.