✦ LIBER ✦

Transformation of rat 3Y1 cells by human papillomavirus type-18 DNA

✍ Scribed by Sumie Watanabe; Kunito Yoshiike

Publisher: John Wiley and Sons
Year: 1988
Tongue: French
Weight: 572 KB
Volume: 41
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910410622

No coin nor oath required. For personal study only.

✦ Synopsis

Rat 3Y I cell cultures transfected with human papillomavirus type-I8 (HPV 18) D N A produced foci of morphologically transformed cells capable of anchorage-independent growth. Analyses of the transformed cell clones obtained after transfection with EcoRI-cleaved linear HPV 18 D N A showed that, although the viral DNAs integrated in the cell D N A had rearrangements (probably around the sites of integration), they appeared to retain the putative transcriptional control region and the downstream area containing E6E7 open reading frames (ORF) intact, and to be transcriptionally active. The expression plasmids constructed from an SV40-derived expression vector (pSV2-0) and HPV 18 D N A fragments containing either E6 or E7 ORF intact were examined for their transforming capacity. HPV 18 E7 ORF was found to encode a transforming gene for rat 3Y I cells.

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