High frequency transformation of Bacillu
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Chang, Shing ;Cohen, Stanley N.
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Article
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1979
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Springer
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English
β 449 KB
A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra