𝔖 Bobbio Scriptorium
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Transformation of plasmid DNA into Streptomyces at high frequency

✍ Scribed by BIBB, MERVYN J.; WARD, JUDITH M.; HOPWOOD, DAVID A.


Book ID
109710224
Publisher
Nature Publishing Group
Year
1978
Tongue
English
Weight
433 KB
Volume
274
Category
Article
ISSN
0028-0836

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High frequency transformation of Bacillu
✍ Chang, Shing ;Cohen, Stanley N. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra